Abstract: The first large-scale (1,200 gpm capacity), sulfate-reducing; bioreactor (SRBR) was constructed in 1996 to treat water from an underground lead mine in Missouri. Other large-scale SRBR systems have been built elsewhere since then. This technology holds much promise for economically treating heavy metals and has progressed steadily from the laboratory to industrial applications. Scale-up challenges include: designing for seasonal temperature variations, minimizing short circuits, changes in metal loading rate s, storm water impacts, and resistance to vandalism. However, the biggest challenge may be designing for the progressive biological degradation of the organic substrate and its effects on the hydraulics of the SRBR cells.