Abstract: A Mn-oxidizing fungus was isolated from a constructed wetland of Hokkaido (Japan), which is receiving the Mn-impacted drainage, and genetically and morphologically identified as Paraconiothyrium sp.-like strain. The optimum pHs were 6.45-6.64, where is more acidic than those of previously reported Mn-oxidizing fungi. Too much nutrient inhibited fungal Mn-oxidation, and too little nutrient also delayed Mn oxidation even at optimum pH. In order to achieve the oxidation of high concentrations of Mn like mine drainage containing several hundreds g-m(-3) of Mn, it is important to find the best mix ratio among the initial Mn concentrations, inocolumn size and nutrient concentration. The strain has still Mn-tolerance with more than 380 g-m(-3) of Mn, but high Mn(II) oxidation was limited by pH control and supplied nutrient amounts. The biogenic Mn deposit was poorly crystallized birnessite. The strain is an unique Mn-oxidizing fungus having a high Mn tolerance and weakly acidic tolerance, since there has been no record about the property of the strain. There is a potentiality to apply the strain to the environmental bioremediation.